高性能

散射光低至24 nm，荧光实现单分子检测

Side Scatter

Nano-flow Cytometry is super-sensitive, which allows label free analysis of single silica and gold nanoparticles down to 24 nm and 7 nm, respectively. For viruses, it is able to detect the signals from single MS2 virus (27 nm). Nano-flow cytometry shows a resolution comparable to that of TEM and the throughput of flow cytometric analysis.

Fluorescence

The fluorescent signals from single R-phycoerythrin molecules can be acquired individually by nano-flow cytometry, which means the NanoFCM is able to study EV proteins as low as ONE copy.

High-speed & High-thoughput

Hundreds of nanoparticles can be detected one by one per second, and a highly representative statistical distribution of particle properties can be obtained in 1 minute.

远离假数据

原始数据实时显示、单颗粒检测方法

The real-time burst traces are monitored/collected to ensure the particles are detected individually. The state of the probe volume is monitored in real time.

无需标记

利用散射光直接检测粒径和浓度

Measure the intrinsic side scatter signal directly from the particles, there is no need to label the particles to get the size and concentration information. The physical properties are acquired without manipulating samples.

灵活实用

方法通用、速度快，无需专有试剂，兼容所有的标记物

Users can choose their proteins of interest (universal antibodies, aptamers) and conjugated dyes freely, the illuminating time is in the range of millisecond, there is no need to worry about the photobleaching. Small sample volume requirements：Sample injection speed down to ~ nL/min level, basically no sample consumption, and the sample can be recycled.

尺寸分布

高精度统计的颗粒大小及分布，团聚分析

Compared with polystyrene beads (RI=1.59), in nFCM system monodisperse silica nanoparticles (RI=1.46) are employed as the reference to calibrate the size of EVs. The resolution of NanoFCM is comparable to TEM. Median, mean, and standard deviation value of a sample can be read from the software, parameter of gated population within a sample is also available.

颗粒浓度

总群和任一亚群的颗粒浓度及比率

In NanoFCM system, the detection efficiency is 100%, the particle concentration is determined via single particle enumeration. The particle counts collected within given time, particle concentration of whole population as well as subpopulation can be determined,  e.g. the ratio of positive. The biochemical function of a subgroup is linked with its size distribution.

生化表征

膜结构，表面蛋白，膜内蛋白，核酸含量和定位分析

After labeling with fluorescent probes, the fluorescence signals of EVs can be detected concurrently with side scatter. By offering quantitatively analysis of single EVs within a sample, the copy number of proteins, payload of engineered EVs can be evaluated individually.

共定位分析

在单颗粒水平实现核酸、膜蛋白、内部蛋白、质膜等组分间的共定位分析

Multiple parameters, including various surface proteins, nucleic acids, lipids and so on can be achieved within a sample simultaneously.